The Effect Of Alkaline Ph On Reaction Rate Of The

The Effect of Alkaline pH on Reaction site of the Enzyme Catecholase Introduction Enzymes atomic number 18 essential to the metabolism of biological organisms. Several factors derriere influence the answer put of enzymes: temperature, pH, enzyme tightness and substrate concentration (Ainsley, 2010). Catecholase, an enzyme in the tyrosinase/polyphenol oxidase family, is responsible for the browning seen in potatoes, apples and mushrooms after they are cut (Capworth, 1998). These fruits and vegetables contain minuscular amounts of catechol, along with the enzyme catecholase, that reacts with oxygen in the atmosphere and forms the brown abstruse benzoquinone (Hemelstine, 2004). This answer arouse be delayed or retained in fruits and vegetables by the assenting of acids, such as lemon juice, and by cooking (Hemelstine, 2004). In this experiment, the effect of pH on the rate of reaction of the enzyme catecholase pass on be examined. Since it has been found previously th at acid can subdue this enzyme (Hemelstine, 2004), this orbit will examine the effects of alkaline pH on enzyme activity. The prediction of this study is that as pH increases (becomes to a greater extent basic), catecholase reaction rate will decrease. Materials and Methods Catecholase activity was persistent as described by Ainsworth et. al.

(2010) in the BI 112 science lab Manual. Specifically, tercet reactions were set up using buffers with pH 7, 9 and 11. The reaction with pH 7 was utilise as a control. severally tube contained 1 ml of potato extract, 6 ml of pH buffer, and 3 ml of catechol. Tubes were covered in parafilm and conflate thoroughly as indivi! dually reagent was added. For each tube, catechol was added conk to prevent the reaction from beginning prior to data collection, and each reaction was conducted in triplicate. To determine the amount of benzoquinone produced, a rubric chart corresponding to benzoquinone concentration (Figure 1) was used (Ainsworth, 2010). quantify began when catechol was added (Time = 0) and measurements were taken at one...If you want to get a broad essay, order it on our website: OrderCustomPaper.com

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